Jean N. DaSilva
Jean N. DaSilva
Office: 613-798-5555 ext. 19704
Work E-mail: email@example.com
Research: Imaging blood flow, metabolism and signal transduction pathways with positron emission tomography
Positron emission tomography (PET) is an imaging technique using radioactive physiological tracers as molecular probes that can be used to study non-invasively cellular pathways that regulate cardiovascular function in normal and disease states in living organisms with high resolution. Our group works on various projects using radioligands for measuring blood flow, metabolism and neurohormonal pathways in cardiovascular disorders, and diabetes. Current field of interests are focused on imaging: A) glucose, fatty acid and oxidative myocardial metabolism; B) the noradrenergic signaling pathway at the transporter, receptors, and cAMP-mediated signal transduction; C) the renin-angiotensin system at the AT1 receptor level in heart, brain and kidney; D) stem cell labeling. The pharmacological binding profile of novel tracers will be evaluated in vitro and in vivo to assess their potential as quantitative markers in animal models of disease states. Our experimental approaches include: 1) radiosyntheses; 2) in vitro binding and autoradiographic studies; 3) biodistribution studies; 4) in vivo binding specificity and selectivity; 5) pharmacological modulation studies in animal models; 6) metabolite and dosimetry studies; 7) large animal PET studies; 8) pharmacokinetic modeling; 9) effect of therapeutic agents on targeted site; 10) PET scans in humans. Development and evaluation of these probes for imaging altered systems provides insights into understanding cardiovascular pathophysiology, help direct and guide therapy, and evaluate progression of disease/treatment using PET. Our research is enhanced by collaborations with various Investigators at the University of Ottawa.
cardiac-brain interaction, neuroreceptors, signal transduction