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Model
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Zeiss AxioImager M2 [Droit]
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Spécifications du microscope
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- Entièrement motorisé: objectifs, cube filtres, condensateur et contrôleur du trajet lumineux
- Platine motorisée (z)
- Platine manuelle (x, y)
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Logiciel
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Zen2009
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Utilisations
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- DIC with confocal acquisition
- Confocal optical sectioning
- Live cell imaging
- Multi-channel imaging
- Timelapse
- Z-stack
- Tile scanning
- FRAP, FLIP, FRET, photo-conversion
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Objectifs
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- 10x, 0.3 NA, Air, EC Plan-Neofluar (DIC)
- 20x, 0.8 NA, Air, Plan-Apo (DIC II)
- 40x, 1.3 NA, Oil, EC Plan-Neofluar (DIC III)
- 40x, 0.8 NA, Water, Achroplan (DIC III)
- 63x, 1.4 NA, Oil, Plan-Apo (DIC III)
- 63x, 1.0 NA, Water, Plan-Apo (DIC III)
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Source lumineuses
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- X-Cite 120 Mercury lamp
- Lampe Halogene
- 6 laser lines: 458nm, 477nm, 488nm, 514nm, 543nm, 633nm
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Filtres confocale
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- Main beam splitter between Ch2 and Ch3: 490nm, 515nm, 545nm
Ch2 Filtres
- LP 420nm
- BP 420nm-480nm
- LP 505nm
- BP 505nm-530nm
- BP 505nm-550nm
Ch3 Filtres
- BP 505nm-530nm
- BP 505nm-550nm
- BP 560nm-615nm
- LP 615nm
- LP 655nm
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| Filtres epifluorescence |
- Bleu Ex G365nm, FT 395nm, Em 420nm LP
- Cyan Ex 436/20nm, FT 455nm, Em 480/40nm
- Vert Ex 470/40nm, FT 495nm, Em 525/50nm
- Jaune Ex 500/20nm, FT 515nm, Em 535/30nm
- Rouge Ex 560/40nm, FT 585nm, Em 630/75nm
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Detecteurs
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3 PMTs
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Local
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RGN 4502
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