Zeiss LSM510 AxioImager M1

The CBIA Core offers access to a variety of confocal microscope systems for high resolution optical sectioning and 3D imaging of samples.

Microscope specifications


Stand model	Zeiss AxioImager M2 [upright]
Microscope controls	Fully automated objective nosepiece, reflector turret, condenser and light path control Manual stage control (x,y) Motorized focus control (z)
Software	Zen2009
Capabilities	DIC with confocal acquisition Confocal optical sectioning Live cell imaging Multi-channel imaging Timelapse Z-stack Tile scanning FRAP, FLIP, FRET, photo-conversion
Objectives	10x, 0.3 NA, Air, EC Plan-Neofluar (DIC) 20x, 0.8 NA, Air, Plan-Apo (DIC II) 40x, 1.3 NA, Oil, EC Plan-Neofluar (DIC III) 40x, 0.8 NA, Water, Achroplan (DIC III) 63x, 1.4 NA, Oil, Plan-Apo (DIC III) 63x, 1.0 NA, Water, Plan-Apo (DIC III)
Light sources	X-Cite 120 Mercury lamp Halogen lamp 6 laser lines: 458nm, 477nm, 488nm, 514nm, 543nm, 633nm
Confocal filters	Main beam splitter between Ch2 and Ch3: 490nm, 515nm, 545nm Ch2 Filters LP 420nm BP 420nm-480nm LP 505nm BP 505nm-530nm BP 505nm-550nm Ch3 Filters BP 505nm-530nm BP 505nm-550nm BP 560nm-615nm LP 615nm LP 655nm
Epifluorescence filter sets	Blue Ex G365nm, FT 395nm, Em 420nm LP Cyan Ex 436/20nm, FT 455nm, Em 480/40nm Green Ex 470/40nm, FT 495nm, Em 525/50nm Yellow Ex 500/20nm, FT 515nm, Em 535/30nm Red Ex 560/40nm, FT 585nm, Em 630/75nm
Detectors	3 PMTs
Location	RGN 4502