The following reagents are produced by the GEM core and distributed through the Biobar in Roger Guindon Hall (RGN).

Booking with Infinity

You can purchase items through Infinity by following these steps:

  1. Select GEM facility.
  2. Select Request services.
  3. Select Order form for GEM Bio-Reagents.
  4. Once the form is submitted, print it (button bottom left) and bring it to the Biobar.

Biobar Reagents Catalogue

Catalog #; Name (permission required)Description
R-C01; LumiPure ECL chemiluminescent detection reagent 200 mlLumiPure ECL chemiluminescent detection reagent offers very low background signal compared to commercially available alternatives.
R-C01; LumiPure ECL chemiluminescent detection reagent 200 mlePEI is a chemically-enhanced version of PEI. This reagent is used to transfect mammalian cells.
R-D01; 2X Isothermal DNA Assembly MixThis kit is used to join two or more DNA fragments using short overlapping regions at the ends of each fragment. Useful in cloning, it replaces commercially available joining mixes. 
R-M01; Mycoplasma testing kit – 20 testsThis kit is used to detect mycoplasma by PCR with low hands-on time. It’s able to detect mycoplasma with as little as 2ul of cell culture media
R-E01; Electroporation Buffers 1 (2 ml)

Neucleofection is a preferred method to perform mammalian cell transfection for cells that are hard to transfect using traditional methods.

These buffers can be used as a substitution for commercial nucleofection buffers. They are compatible with Neucleofector 1 or 2B but not 4D.

The following 7 Nucleofector 2B programs are suggested: A-020, T-020, T-030, X-001, X-005, L-029 and D-023.

R-E02; Electroporation Buffers 2 (2 ml)
R-E03; Electroporation Buffers 3 (2 ml)
R-E04; Electroporation Buffers 4 (2 ml)
R-E05; Electroporation Buffers 1-4 (4 x 1 ml)

Contact us

Genome Editing and Molecular biology (GEM) Facility

If you have any questions, please contact:

James Taylor
Email: [email protected]