The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

Zeiss AxioImager M2 microscope
 
Stand modelAxioImager M2 [upright]
Microscope controls
  • Fully automated objective nosepiece, reflector turret and condenser
  • Fully motorized focus control (z) and stage control (x, y)
  • Manual light path control
SoftwareZen Blue 2.3
Capabilities
  • Epifluorescence imaging
  • Brightfield, phase contrast, DIC
  • Multi-channel imaging
  • Z-stack (with Extended focus module)
  • [retired function: Tiling (MosaiX) 2024 Jan]
Objectives
  • 5x, 0.16 NA, Air, EC Plan-NeoFluar (BF, DF)
  • 10x, 0.45 NA, Air, Plan-Apo (BF, DIC II, DF)
  • 20x, 0.80 NA, Air, Plan-Apo (BF, DIC II, DF)
  • 40x, 1.3 NA, Oil, EC-Plan-NeoFluar (BF, DF)
  • 63x, 1.4 NA, Oil, Plan-Apo (BF, DF)
Light sources
  • X-Cite XYLIS LED
  • Transmitted LED source
Filters
  • Blue Ex 390/22nm, FT 420nm, Em 460/50nm
  • Green Ex 470/20nm, FT 495nm, Em 517/25nm
  • Yellow Ex 500/25nm, FT 515nm, Em 535/30nm
  • Red Ex 560/40nm, FT 585nm, Em 630/75nm
  • Far RedEx 640/30nm, FT 660nm, Em 690/50nm
Detectors
  • AxioCam mRm CCD (monochrome)
  • AxioCam mRc (colour)
LocationRGN 3164A