The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

Leica DMI 6000

Stand model

Leica DMI 6000 [Inverted]

Microscope controls

  • Fully automated objective nosepiece, reflector turret and condenser
  • Fully motorized stage control (x, y), focus control (z) and light path control

Software

Volocity 7.0

Capabilities

  • Epifluorescence imaging
  • Brightfield, phase contrast, DIC
  • Multi-channel imaging 
  • Timelapse
  • Z-stack
  • Tiling and Multi-position (Unique: 4 slides holder)

Objectives

  • 10x HC Plan FL 0.3 Ph1-/D11
  • 20x HCX Plan FL L 0.4 Corr Ph1, 0-2/C
  • 40x HCX Plan FL L 0.6 Corr Ph2, 0-2/C
  • 40x HCX Plan Apo, 1.25-0.75 Oil 0.17/D
  • 63x HCX Plan Apo, 1.4-0.6 Oil C
  • 100x HCX Plan Apo, 1.4-0.7 Oil CS

Light sources

Transmitted light source: Halogen

Fluorescence light source: Excelitas X-Cite Mini LEDs

Filters

  • Blue Ex: G365, FT: 395 nm, EM: BP 445/50
  • Green Ex: BP 450/50, FT: 480, EM: BP 510/50
  • Red Ex: BP 550/25, FT: 570, EM: BP 605/70

Camera

ORCA-ER (CCD)

Location

RGN 4502