The CBIA Core is home to a variety of wide field epifluorescent microscopes to suit routine imaging needs in both live and fixed samples.

Microscope specifications

ThermoFisher EVOS FL Auto 2 microscope
 

Stand model

ThermoFisher FL Auto 2 (inverted)

Microscope controls

Fully automated objective nosepiece, reflector turret and condenser, focus control (z) and stage control (x, y).

Software

Auto2

Capabilities

  • Epifluorescence, Brightfield and phase contrast imaging.
  • Z-stack Tiling, time lapse, Live cell, autofocus
  • Various stage adaptors for slides, plates and flasks

Objectives

  • 5x, 0.13 NA, Air, PL FL LWD PH
  • 10x, 0.25 NA, Air, PL FL LWD PH
  • 20x, 0.40 NA, Air, PL FL LWD PH
  • 40x, 0.6 NA, Air, LD
  • 60x, 1.42 NA, Oil, PL APO

Light sources

LED light sources

Filters
  • Blue          Ex 357/44nm,Em 447/60nm
  • Green        Ex 470/22nm,Em 510/42nm
  • Red          Ex 585/29nm,  Em 624/40nm
  • Far Red     Ex 628/40nm,  Em 692/40nm

Detectors

Colour and Monochrome camera

Location

RGN 3164A