BD LSRFortessa – 16-colour Analyzer

The FCV Core Facility currently houses the following instruments: BD LSRFortessa SORP, BD Celesta, Beckman Coulter MoFlo Astrios, and an AutoMACS Pro Magnetic Cell Separator (located at OHRI).

Instrument specifications

300mW – 488nm (Blue) solid state
50mW - 561nm (Yellow-Green) solid state
40mW – 640nm (Red) solid state
50mW – 405nm (Violet) solid state

  • High-Throughput Sampler for 96-well and 384-well plates
  • 12x75mm 5ml polystyrene tubes (Falcon 352052)
Fortessa Configuration: 4 Lasers – 16 colours
Fortessa Configuration: 4 Lasers – 16 colours

Configuration
 

Laser

A

B

C

D

E

F

G

H

405nm

N/A

780/60: BV786, QDot800

710/50: BV711, QDot705

660/20: BV650, QDot655

N/A

610/20: BV605, QDot605

530/30: BV510, V500, QDot525

450/50: Pacific Blue, BV421,V450

488nm

695/40: PerCP-Cy5.5

530/30: FITC, eGFP, BB515, AF488

SSC

N/A

N/A

N/A

N/A

N/A

561nm

780/60: PE-Cy7

710/50: PE-Cy5.5

670/30: PE-Cy5

610/20: mCherry, PE-CF594

586/15: PE, Ds-Red, PI

N/A

N/A

N/A

640nm

780/60: APC-Cy7, APC-H7, APC-Fire750

730/45: AF700

660/20 APC, AF647

N/A

N/A

N/A

N/A

N/A