Resources

Introduction to Flow Cytometry

• Online Tutorial

Antibody Panel Design Tools

• Fluorofinder
• BD Horizon Guided Panel Solution (GPS)
• Table of Fluorochromes

Sample Preparation Protocols

• References for Tissue Dissociation:
  • Worthington Tissue Dissociation Guide
• Protocols for Sample Preparation:
  • Current Protocols in Immunology: Unit 5.3 Preparation of Cells and Reagents for Flow Cytometry
  • AbD Serotec
  • eBiosciences (PDF, 57KB)
  • Santa Cruz
  • Cell Signaling
  • Stemcell

Small Particle Flow Cytometry

• EV Flow Cytometry Working Group
  • MIFlowCyt-EV (Minimum Information about a Flow Cytometry experiment for EVs)
    • Summary Handout (CDW2019)
    • Framework (PDF, 34KB) of procedures and sample controls
• Software for Light Scatter Calibration
  • FCMpass
  • Rosetta Calibration

Flow Cytometry Analysis Programs & Tutorials

• Kaluza
• FlowJo Tutorials
• Introduction to FlowJo (PDF, 15.5MB)

Open-source Software:

• Flowing Software