Leica Thunder Imager

The Leica Thunder Imager is an upright microscope for fluorescence, transmitted light, and true color imaging. This instrument is equipped with Leica’s computational clearing and deconvolution to remove out-of-focus blur, enhance contrast, and sharpen details. The software Navigator function can be used for rapidly generating sample overviews for subsequent selection of areas of interest.

Instrument specifications

Stand model	Upright Stand Leica DM6 B
Microscope controls	•Fully Automated objective nosepiece, reflector turret and light path control •Fully motorized stage (x,y) and focus control (z)
Software	Leica Application Suite X (LAS X) with Leica's thunder algorithms is capable of Instant Computation Clearing (ICC), Small Volume Computation Clearing (SVCC) for cells, yeast, and other thin samples, and Large Volume Computational Clearing (LVCC) for tissue sections, embryos, organoids, and other thick samples.
Capabilities	Widefield system for image acquisition, processing, and quantification. Multi-channel imaging Time series Z-stack Tile scanning (navigator)
Objectives	1.25×/0.04 HC PL Fluotar \| DRY objective 5×/0.12 N PLAN \| DRY objective 10×/0.32 HC PL Fluotar \| DRY objective 40×/0.95 HC PL Apo CORR \| DRY objective 20×/0.75 HC PL Apo IMM CORR CS2 \| Multi-Immersion 63×/1.40-0.6 HC PL Apo Oil \| OIL Immersion
Fluorescence / Reflected light Source & Filter Sets	Fluorescent Light source: EL6000 Filter name \| excitation \| Dichroic Mirror \| Emission DAPI 350/50 400 460/50 GFP 470/40 495 525/50 Cy3 545/25 565 605/70 Texas Red 560/40 585 630/75 Cy5 620/60 660 700/75 Cy7 710/75 760 810/90
Transmitted Light & Contrasting Method	Transmitted light source: LED Brightfield
Camera	Leica K5 sCMOS monochrome camera, 2048 x 2048 pixels (for fluorescence imaging) Leica Flexacam C3 Camera, 12 MP CMOS sensor (for histology dyes)
Location	RGN 3142