Common Equipment - CETS | Faculty of Medicine

Scientist loads sample onto low pedestal of Nanodrop in laboratory — Faculty of Medicine

Core Facilities- CF

Common Equipment and Technical Services (CETS) - CF

Common Equipment

Equipment Usage Rules

Shared instruments identified by a yellow sign with the letter “T” require mandatory training. To request a training, complete the CETS Request Form. This rule applies to the following instruments:
- Centrifuges and rotors
- qPCR (CFX)
- Covaris Sonicator
- Lyophilizer
- SpeedVac
- ddPCR
- Injection module of the Bio-Tek plate reader
- Hypoxia Chamber
- Fraction Collector

Shared instruments identified by a red sign with the letter “B” must be booked online through Infinity
Read all signs and instructions on or near the instruments
Sign the logbooks (yellow duo-tang) every time you use an instrument
Use only the Research Drive or email to export data from shared instruments (no USB sticks allowed)

Equipment list

Ultracentrifuges

Ultracentrifuges
Name	Description	Max Speed	Volumes	Location
L-100XP (2x)	Beckman floor model. Account creation required.	100,000 rpm (802,400 x g)	5 to 50 mL	RGN4522, RGN4172C
Optima XPN-100 (2x)	Beckman floor model. Account creation required.	100,000 rpm (802,400 x g)	5 to 50 mL	RGN3502, RGN4258

Ultracentrifuges-MAX

Ultracentrifuges-MAX
Name	Description	Max Speed	Volumes	Location
Optima MAX-Micro	Beckman tabletop model	130,000 rpm (1,019,000 x g)	0.5 to 8 mL	RGN3156
Optima MAX-XP	Beckman tabletop model. Account creation required.	130,000 rpm (1,019,000 x g)	0.5 to 8 mL	RGN4258

High Speed Centrifuges

High Speed Centrifuges
Name	Description	Max Speed	Volumes	Location
Avanti J-25 (3x)	Beckman floor model	25,000 rpm (75,600 x g)	15 to 500 mL	RGN2541, RGN3121, RGN4172C
J-20XPI	Beckman floor model	20,000 rpm (51,500 x g)	15 to 500 mL	RGN4200B
JXN-26	Beckman floor model. Account creation required.	26,000 rpm (81.770 x g)	15 to 500 mL	RGN3502

Low Speed Centrifuges

Low Speed Centrifuges
Name	Description	Max Speed	Volumes	Location
J6-MI	Beckman floor model + rotor JS-4.2	4,200 rpm (5,020 x g)	15 to 1000 mL	RGN4200B
Sorvall Legend XFR	ThermoFisher floor model + 3 rotors Thermo	15,000 rpm (24,652 x g)	15 to 50 mL + plaques	RGN3121
Sorvall ST 16R	ThermoFisher tabletop model + 3 rotors Thermo	13,000 rpm (15,777 x g)	15 to 50 mL + plates	RGN3121
Eppendorf 5810R	Eppendorf tabletop model + rotor A-4-81	4,000 rpm (3,220 x g)	15 to 50 mL + plates	RGN4258
Qiagen Sigma 4-15C	Qiagen Sigma tabletop model + rotor "SW microplate"	6,200 rpm (5,788 x g)	Plates	RGN3156

Rotors Ultra

Rotors Ultra
Name	Description	Max Speed	Volumes	Location
Type 45Ti (2x)	Specification sheet for fixed-angle Beckman rotor "Type 45Ti"	45,000 rpm / 235,000 g	6 x 50 mL	RGN4176, RGN3156
Type 50.2Ti	Specification sheet for fixed-angle rotor Beckman "Type 50.2Ti" Aluminium spacer for Quick-Seal tubes available (Cat.#: 342699)	50,000 rpm (302,000 x g)	12 x 39 mL	RGN4176
Type 70Ti	Specification sheet for fixed-angle rotor Beckman "Type 70Ti" Aluminium spacer for Quick-Seal tubes available (Cat.#: 342699)	70,000 rpm (504,000 x g)	8 x 39 mL	RGN4176
Type 70.1Ti	Specification sheet for fixed-angle rotor Beckman "Type 70.1Ti"	70,000 rpm (450,000 x g)	12 x 13.5 mL	RGN4176
SW32Ti (3x)	Specification sheet for swinging-bucket rotor Beckman "SW32Ti"	32,000 rpm (175,000 x g)	6 x 38.5 mL	RGN4176, RGN3156
SW40Ti	Specification sheet for swinging-bucket rotor Beckman "SW40Ti"	40,000 rpm (285,000 x g)	6 x 14 mL	RGN3156
SW41Ti (3x)	Specification sheet for swinging-bucket rotor Beckman "SW41Ti"	41,000 rpm (288,000 x g)	6 x 13.2 mL	RGN4176, RGN3156
SW55Ti	Specification sheet for swinging-bucket rotor Beckman "SW55Ti"	55,000 rpm (368,000 x g)	6 x 5 mL	RGN4176

Rotors Ultra-Max

Rotors Ultra-Max
Name	Description	Max Speed	Volumes	Location
MLA-80	Specification sheet for fixed-angle rotor Beckman "MLA-80"	80,000 rpm (444,000 x g)	8 x 8 mL	RGN3156
MLA-130	Specification sheet for fixed-angle rotor Beckman "MLA-130"	130,000 rpm (1,019,000 x g)	10 x 2 mL	RGN3156
TLA-100.3 (2x)	Specification sheet for fixed-angle rotor Beckman "TLA-100.3" Delrin tube adaptors available (Cat.#: 355919)	100,000 rpm (541,000 x g)	6 x 3.5 mL	RGN4176, RGN3156
TLA-120.1	Specification sheet for fixed-angle rotor Beckman "TLA-120.1"	120,000 rpm (627,000 x g)	14 x 0.5 mL	RGN3156

Rotors High Speed

Rotors High Speed
Name	Description	Max Speed	Volumes	Location
JLA-10.500	Specification sheet for fixed-angle rotor Beckman "JLA-10.500"	10,000 rpm (18,600 x g)	6 x 500 mL	RGN3156
JA-10	Specification sheet for fixed-angle rotor Beckman "JA-10"	10,000 rpm (17,700 x g)	6 x 500 mL	RGN4176
JLA-16.250 (2x)	Specification sheet for fixed-angle rotor Beckman "JLA-16.250"	16,000 rpm (38,400 x g)	6 x 250 mL	RGN4176, RGN3156
JA-25.50 (4x)	Specification sheet for fixed-angle rotor Beckman "JA-25.50"	25,000 rpm (75,600 x g)	8 x 50 mL	RGN4176, RGN3156, RGN2541
JA-20.1	Specification sheet for fixed-angle rotor Beckman "JA-20.1"	20,000 rpm (51,500 x g)	32 x 15 mL	RGN3156
JA-25.15	Specification sheet for fixed-angle rotor Beckman "JA-25.15"	25,000 rpm (75,600 x g)	24 x 15 mL	RGN4176

Rotors Low Speed

Rotors Low Speed
Name	Description	Max Speed	Volumes	Location
JS-4.2	Swinging-bucket rotor Beckman compatible with centrifuge J6-MI	4,200 rpm (5,020 x g)	15 to 1000 mL	RGN4200B
Thermo TX 400	Swinging-bucket rotor Thermo compatible with tabletop centrifuge Sorvall ST 16R	5,000 rpm (4,696 x g)	15 to 50 mL	RGN3121
Thermo Fiberlite BioSEAL F15-6X 100y	Fixed-angle rotor Thermo compatible with centrifuge Sorvall ST 16R and Sorvall Legend XFR	15,000 rpm (24,652 x g)	15 to 50 mL	RGN3121
Thermo M20	Swinging-bucket rotor Thermo compatible with centrifuge Sorvall ST 16R and Sorvall Legend XFR	4,000 rpm (2,272 x g)	Plates	RGN3121
Thermo BIOLiner	Swinging-bucket rotor Thermo compatible with centrifuge Sorvall Legend XFR	3,500 rpm (2,739 x g)	15 to 50 mL	RGN3121
Eppendorf A-4-81	Swinging-bucket rotor Eppendorf compatible with centrifuge 5810R	4,000 rpm (3,220 x g)	15 to 50 mL + plates	RGN4258
Qiagen Sigma SW microplate	Swinging-bucket rotor Qiagen Sigma compatible with centrifuge 4-15C	6,200 rpm (5,788 x g)	Plates	RGN3156

Imaging Systems
Name	Description	Location
ChemiDoc MP (2x)	Bio-Rad imager for detection of chemiluminescence, fluorescence (400 to 800 nm) and stain-free technology. White and blue sample trays available.	RGN4176, RGN3156
ChemiDoc basic 2x)	Bio-Rad imager for detection of chemiluminescence and stain-free technology. White and blue sample trays available.	RGN3502, RGN2541
LI-COR	Odyssey FC model for detection of chemiluminescence and fluorescence (600 to 800 nm).	RGN3156
LAS4000	GE ImageQuant imager for detection of chemiluminescence and visible RGB fluorescence.	RGN3502
GelDoc XR+ (2x)	Bio-Rad imager for UV and white light transillumination. For EtBr gels.	RGN4258, RGN3156
Film Developer	Model Konica SRX-101A.	RGN4262B
UV Transilluminator (2x)	For visualization of DNA and proteins with UV light.	RGN4258, RGN3520
Blue Light Transilluminator (2x)	Model BluPAD from FroggaBio. For visualization of DNA and proteins with blue light (e.g.: Sybr SAFE, GelGreen)	RGN4258, RGN3125
ZOE Imager (2x)	Fluorescent cell imager inverted system with brightfield and 3 channels (red, green and blue). Suitable for routine cell culture.	RGN4176, RGN3125

PCR Machines
Name	Description	Location
CFX Opus 96 (2x)	Bio-Rad real-time PCR system capable of capturing fluorescence on 5 different channels (450-730 nm) in low-profile 96-well plates. Also features a FRET channel. Reference names = Petrie Island and Britannia 2.0.	RGN4176, RGN3156
CFX 96	Bio-Rad real-time PCR system capable of capturing fluorescence on 5 different channels (450-730 nm) in low-profile 96-well plates. Also features a FRET channel. Reference name = Mooney's Bay	RGN3502
CFX 384	Bio-Rad real-time PCR system capable of capturing fluorescence on 4 different channels (450-690 nm) in low-profile 384-well plates. Also features a FRET channel. Reference name = Sandbanks	RGN3156
ddPCR	Bio-Rad QX200 Digital Droplet PCR system for ultra-sensitive nucleic acid detection and absolute quantification. The system includes: 1) QX200 droplet generator, 2) PX1 plate sealer, 3) C1000 Touch thermal cycler and 4) QX200 droplet reader.	RGN4124
Eppendorf MasterCycler	Eppendorf MasterCycler EpGradient thermal cycler with 96 wells.	RGN4176
ProFlex PCR (2x)	ThermoFisher thermal cycler containing 3 independent blocks of 32 wells each.	RGN4258, RGN3125

Spectrophotometers and Plate Readers
Name	Description	Location
Nanodrop 2000 (2x)	ThermoFisher pedestal spectrophotometer for measuring DNA, RNA and protein concentrations in samples of 2 µL or more.	RGN4176, RGN3156
Synergy H1	BioTek Synergy H1 Multi-mode plate reader for measuring absorbance (230-999 nm), fluorescence (300-700 nm) and luminescence in 6 to 384-well plates. Temperature control between 21ºC and 45ºC and shaking option.	RGN4176
Synergy H1 + injecteurs + Take3	BioTek Synergy H1 Multi-mode plate reader for measuring absorbance (230-999 nm), fluorescence (300-700 nm) and luminescence in 6 to 384-well plates. Temperature control between 21ºC and 45ºC and shaking option. Also features a dual injection module. Compatible with the Take3 microvolume plate, for measuring DNA, RNA or protein concentration in samples of 2 uL or more.	RGN3156
PowerWave XS2	BioTek plate reader for measuring absorbance (200-999 nm) in 6 to 384-well plates.	RGN4522F
GloMax 96	Promega luminometer with dual injection module, for measuring luminescence in 96-well plates.	RGN4522F
UV-3100PC	VWR UV-Vis spectrophotometer with cuvettes for measuring absorbance between 190-1100 nm.	RGN3226

Drying Systems
Name	Description	Location
Lyophiliser Labconco	Model FreeZone 72040 4.5 L -105ºC Series Default setup = Lyophiliser + Small Tray Chamber. Additional options: 1) Lyophiliser + 8-ports Manifold, 2) Lyophiliser + Mini-stoppering chamber. Contact [email protected] to change this setup. Contact us to check compatibility of evaporating solvents.	RGN3121
SpeedVac Labconco	Model CentriVap Acid 16108440 + Cold Trap 16109400 capable of holding 0.5mL tubes (72x) and 1.5 mL tubes (60x). Contact us to check compatibility of evaporating solvents. Temperature control between 21ºC and 60ºC.	RGN3121
Nitrogen Evaporator	Model N-EVAP 112 Organomation capable of holding 24 samples between 1.5 and 50 mL.	RGN4176
Drying Oven	Forced air oven model 89511-412. Temperature limits = 50ºC to 250ºC.	RGN4258
Gel Dryer (2x)	Vacuum gel dryer model Bio-Rad 583	RGN3226

Shaker Incubators
Name	Description	Location
Infors (3x)	Multitron shaking incubators with 3 stacked units for glass flasks and tubes. Adjustable temperature between 18ºC and 50ºC. Maximum speed = 250 rpm	RGN4258, RGN3156
Innova 4000	New Brunswick shaking incubator for flasks and tubes. Adjustable temperature between 18ºC and 50ºC. Maximum speed = 250 rpm	RGN2541

Lysis Instruments (Sonicators, dissociators)
Name	Description	Location
Covaris S220	Ultrasonicator Covaris for processing samples from 130 uL or 1 mL. Ideal for cell lysis, DNA/RNA/protein extraction and DNA/RNA/chromatin shearing.	RGN4176
Sonic 60	Fisher Sonic 60 dismembrator for samples from 0.5 to 15 mL.	RGN3226
FB120	Fisher FB120 dismembrator for samples from 0.5 to 15 mL.	RGN3226
MagNA Lyser	Tissue homogenization instrument capable of holding 16 samples.	RGN4258
GentleMACS Octo Dissociator	Tissue homogenizer able to process 8 samples. Heaters also available.	RGN4176

Cell Culture
Name	Description	Location
BSC	Biosafety cabinet (BSC) for cell culture	RGN3174
CO₂ incubator	Cell culture incubator set at 37ºC and 5% CO₂.	RGN3174
Countess II	Invitrogen automated cell counter with fluorescence detection (red and green). Compatible with disposable and reusable slides.	RGN3125
NEX-Cellometer K2	ESBE automated cell counter with fluorescence detection (red and green). Compatible with disposable slides.	RGN4176
Nucleofector 2b	Lonza electroporation instrument for cell culture transfection in cuvettes.	RGN3174
Nucleofector 4D	Lonza electroporation instrument for cell culture transfection. X unit for electroporation in cuvettes and Y unit for electroporation in 24-well plates.	RGN3174
ZOE Imager (2x)	Fluorescent cell imager inverted system with brightfield and 3 channels (red, green and blue). Suitable for routine cell culture.	RGN4176, RGN3125

More instruments available
Name	Description	Location
Scintillation Counter	Tri-Carb 4910TR Liquid Scintillation Counter capable of detecting alpha, beta and gamma radiation.	RGN3156
Fraction Collector	Brandel density gradient fractionation system (tube piercing system with pump, UA-6 detector with 254 and 280nm filters, Foxy R1 fraction collector). Ideal for studying protein-protein interactions or separating nucleic acids, proteins and organelles.	RGN3502
Hypoxia Chamber Xvivo X3	BioSpherix hypoxia chamber with 4 individual cell culture incubators and a large preparation chamber.	RGN3127
Washer and Dryer	For yeast velvet washing.	RGN4201

Research supplies

Quick Links

Booking with Infinity

Link to the login page

User guide - Infinity

Scientists in a laboratory conducting experiments and analyzing samples

Training request

CETS Request Form