The MCF is here to support you for all of your metabolomics needs, from project conceptualization to publication. We provide assistance with project design, grant applications, running samples, processing data, data analysis and help with interpretation.

2022 pricing list


UofO and TF Clients 

Other academia 

Industrial clients 

LC-QQQ-MS targeted full panel (IPdMRM) 




LC-QQQ-MS targeted partial panel (IPdMRM) 




LC-QTOF-MS metabolite profiling 




Other LC-QTOF-MS targeted  protocols 








Stable Isotope Tracing Analyses 




Additional Staff Time 




Pricing for method development = price per injection + staff time

Please note: Prices are subject to change without notice. Leftover samples are stored for 12 months at -80°C. Invoices are generally issued after the analysis.

Metabolomics profiling/Quantitative metabolomics

The uOttawa metabolomics core facility offers both metabolite profiling and quantification in a given matrix using LC-MS and GC-MS (depending on the compounds chemical nature and ionization efficiency). However, the detection and quantification is highly dependent on the following:  

  • The authentic standards ae available commercially or able to obtain the standards synthetically 

  • The ability to detect the standard using any of the listed instrument 

  • The ability to efficiently extract the compounds in the given matrix and derivatize if necessary (especially for GC/MS) 

  • The ability to have good chromatography of the endogenous compounds to clearly separate them from the confounding metabolites  

uOttawa metabolomics core facility currently possesses more than 200 authentic metabolite standards from various classes or groups including, acyl coenzyme A(s), amino acids and derivatives, carboxylic acids and derivatives, organic acids and derivatives, bile acid(s), carnitine and acyl-carnitine(s), choline and folate pathway metabolites, fatty acid(s) (GC-MS only), glycolysis, pentose phosphate pathway, NAD and tryptophan pathway metabolites, nucleotides, nucleosides and derivatives, polyamines, TCA cycle intermediates and vitamins. We offer pathway/groups based quantitative analysis. We also offer metabolite profiling where we do our best to try to analyze as much as possible from the given set of sample.  

If your desired compounds are not in our list, please make an appointment to talk with us. We offer our expertise to create new methodologies specific to your needs. Method development is time consuming thus custom analysis charges will apply (staff & instrument time). 

Untargeted Metabolomics

Due to limited staff and time, the uOttawa core facility currently only offers data acquisition using our LC-QTOF-MS for the untargeted metabolomics workflow. 

With paying MCF staff time the following steps can be done at MCF: 

  • The acquired data will be in terms of molecular features (including all ions and adducts). 

  • The generated list of extracted metabolites or “features” are subjected to data alignment across different samples, which will be done using Agilent’s Profinder software.  

  • Statistical analysis (Univariate) with Agilent’s Mass Profiler Professional software, will provide a list of differentiating “features” which will be “tagged” for identification/database search for you. We will not be able to help with the identification/database search because of the time factor, however can guide you.  

  • Once the metabolites are putatively/tentatively identified by you, the samples will be re-run along with authentic standards for confirmation in terms of retention time, mass spectrum and MS/MS fragmentation pattern match.  

  • Spiking authentic standards in the sample can further confirm the identity with an increase in the significant metabolite peak intensity along with retention time, mass spectrum and fragmentation pattern. 

Contact us

Metabolomics Core Facility

Shipping address:
Attn: Shama Naz
Metabolomics Core Facility
University of Ottawa
Roger Guindon Hall
451 Smyth Road, Room 1129/1130
Ottawa ON  K1H 8M5

Shama Naz, PhD

Manager, Metabolomics Core Facility
Office: RGN 4108; Core facility: RGN 4520; Lab: RGN 4210B
Phone: 613-562-5800 ext. 8018
[email protected]

Julie St-Pierre, PhD

Professor, Department of Biochemistry, Microbiology and Immunology
Director, Metabolomics Core Facility
[email protected]