The Biological John L. Holmes Mass Spectrometry Facility at the University of Ottawa provides support for high quality research activities and enhances the educational experience of students.


  • Protein identification in complex mixtures (biomarker discovery)
  • Post-translational modification analysis (phosphorylation, N-acetylation, glycosylation etc.)
  • Quantitative proteomics
    • MS-based on stable isotope labeling method
    • Label-free (MS1 peak integration/intensity, counting MS2 spectra)
  • Identification of interacting proteins (protein complexes analysis, protein-protein interactions)
  • DNA-, RNA- protein interactions
  • Targeted proteomics (PRMs)
  • Multiple stage fragmentation capabilities: HCD, CID and ETD
  • Proteomic based on MS3 level [tandem mass tag (TMT), Isobaric tags for relative and absolute quantitation (iTRAQ), phosphoproteomics, cross-linked peptides]


  • Mass Determination
  • Natural abundance of isotopes
  • Structural characterization/elucidation (full scan and fragmentation pattern)
  • Quantification
  • Targeted and untargeted metabolomics
  • Protein-ligand interaction


Contact Us

Biological facility

Zoran Minic
[email protected]
Marion Hall - Room 02
Ottawa, ON K1N 1A2
Tel: 613-562-5800 ext. 1626